Feng et al. (2015) recently reviewed the progress in nanopore technology from the past as well as the latest advances. They describe the different types of nanopores and discuss recent and potential applications. Nanopore-based sequencers have the potential to quickly and reliably sequence the entire human genome “for less than $1000, and possibly for even less than $100”. Some significant advantages of nanopores include minimal sample preparation, elimination of the need for amplification or modification (nucleotides, polymerases or ligases), label-free, rapid, ultra-long reads (read lengths of 10,000–50,000 bases), high throughput, and low material requirement. Among the challenges discussed, single base recognition and slowing down the rate of DNA velocity are still the principal challenges for the nanopore rechnology.
Nanopore-based Fourth-generation DNA Sequencing Technology. Feng Y, Zhang Y, Ying C, Wang D, Du C. Genomics Proteomics Bioinformatics. 2015 Feb;13(1):4-16. Review. PMID:25743089
Posted by Yannis Trakadis, MD