Asymmetric dimethylarginine is an inhibitor of endothelial nitric-oxide synthase by competing with l-arginine. In 2008, Sud et al showed that that asymmetric dimethylarginine induces translocation of endothelial nitric-oxide synthase to mitochondria via a protein nitration dependent mechanism, resulting in potential mitochondrial dysfunction (Sud N, Am. J. Physiol. Cell Physiol. 294, C1407–1418). In a more recent article (Rafikov et al. J Biol Chem. 2013 Mar 1;288(9):6212-26.) this same groups offered a mechanism for this protein nitration: eNOS phosphorylation at Akt1-dependent phosphorylation sites.
Since dimethylarginine us easily measurable via GC-MS, this has interesting implications for potential clinical markers to follow in a clinical setting.
Hilary Vernon MD PhD